The aim of this study was to better characterize rabbit proximal kidney tubule cells cultured on collagen IV-coated porous inserts, as compared to the same cells seeded in standard plastic wells. Total protein contents in confluent monolayers on permeable membranes were about twofold higher than those measured in confluent cultures in plastic wells.
Microscopy examinations suggested that such a difference was probably due to a higher cell density and to an impressive development of the apical brush-border membrane. Moreover, measurement of unidirectional transport of p -aminohippuric acid and tetraethylammonium bromide confirmed the high polarization level of cultures on porous inserts. On the other hand, this paper reports for the first time in the literature that GRED and Se-GPX, two phase II detoxification enzymes, were well maintained in cultures of rabbit proximal kidney tubule cells.
Our results show that culturing rabbit proximal kidney tubule cells on collagen IV-coated porous membranes was accompanied by an improvement of both morphological and biochemical properties of the cells. This is a preview of subscription content, log in via an institution to check access. Rent this article via DeepDyve. Institutional subscriptions.
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