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Porous collagen-glycosaminoglycan collagen-GAG scaffolds have shown promising clinical results for wound healing; however, these scaffolds do not replace the dermal and epidermal layer simultaneously and rely on local endogenous signaling to direct healing.
An ideal candidate for this is platelet-rich plasma PRP as it is a natural reservoir of growth factors, can be activated to form a fibrin gel, and is available intraoperatively. This motivated us to develop a strategy to successfully incorporate PRP homogeneously within the pores of the collagen-GAG scaffolds. In addition, the composite scaffold had enhanced mechanical properties when compared to PRP gel alone , while providing a continuous upper surface of extracellular matrix ECM for keratinocyte seeding.
The levels of the factors released from the composite scaffold were sufficient to sustain proliferation of key cells involved in wound healing, including human endothelial cells, mesenchymal stromal cells, fibroblasts, and keratinocytes; even in the absence of FBS supplementation. In functional in vitro and in vivo vascularization assays, our composite scaffold demonstrated increased angiogenic and vascularization potential, which is known to lead to enhanced wound healing.
