E-mail: rlu njust. E-mail: boris. A facile and sensitive glucose sandwich assay using surface-enhanced Raman scattering SERS has been developed. Glucose was captured by 3-aminopheyonyl boronic acid APBA modified Ag nanoparticles decorated onto a polyamide surface. APBA forms specific cis -diol compounds with glucose molecules avoiding interference by other saccharides and biomolecules in urine enabling its selective detection.
Additionally, the developed SERS assay was reusable, and its applicability in artificial urine samples demonstrated future clinical utility confirming the potential of this innovative technology as a diagnostic tool for glucose sensing. SERS has been widely applied in the detection of a broad variety of chemical compounds and biomolecules due to its superior Raman signal enhancement capacity, resulting in the development of extremely sensitive molecular sensors at the micro level.
Many studies have been conducted on the detection of glucose using SERS. However, glucose naturally emits weak Raman signals due to its small Raman scattering cross-section, measuring only 5. At present, Raman-label detection is the most frequently employed approach for identifying biological molecular targets.
To overcome this limitation, one method is to utilize carbohydrate recognition molecules, such as boronic acid, 26 to capture glucose molecules onto the nanostructured surface. This increases the concentration of captured glucose, hence improving the detection sensitivity. Boronic acid and its derivatives have been demonstrated to selectively form trans โ cis -diol complexes with glucose molecules in a selective manner.
The quantitative analysis of glucose was performed by SERS functionalized with a variety of boronic acid derivatives. Thus, glucose can form covalent bands in a 1 : 2 ratio with two boronic acids. There is substantial evidence that cyclic borates formed between glucose and boronic acid enable us to perceive glucose more selectively than other saccharides. Malini Olivo et al. This technology was further improved by substituting an alkyne-functionalized boronic acid for the boronic acid, which offers a novel glucose binding mechanism on a highly sensitive SERS substrate, overcoming obstacles associated with detecting specific glucose in bio-fluids.
